Coupled chemo(enzymatic) reactions in continuous flow

• Ruslan Yuryev,
• Simon Strompen and
• Andreas Liese

Beilstein J. Org. Chem. 2011, 7, 1449–1467, doi:10.3762/bjoc.7.169

• . Three different approaches to such reaction systems are presented herein and discussed in view of their advantages and disadvantages as well as trends for their future development. Keywords: biocatalysis; chemo-enzymatic reaction sequences; continuous flow; coupled reactions; reaction cascades

• metabolism, is still a distant goal for any man-made chemical factory. Metabolic pathways, consisting of enzymatic sequential and coupled reactions (Figure 1), lie at the core of any living system and have been optimized by evolution over billions of years to create the phenomenon of life as we know it. In

• hexahistidine tags on nickel–agarose beads (“super beads”) [33]. These enzymes catalyze a complex network of sequential and coupled reactions, in which galactose, uridine monophosphate (UMP) and inorganic polyphosphate are converted to uridine diphospate galactose (UDP-Gal) in the presence of catalytic amounts